Coding

Part:BBa_K156011:Design

Designed by: George McArthur IV   Group: iGEM08_Virginia   (2008-10-28)

aadA (streptomycin 3'-adenyltransferase)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 480
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was codon-optimized for expression in E. coli. The following restriction enzyme sites were also removed: EcoRI, NotI, XbaI, SpeI, PstI, NheI, AvrII, ApoI, MfeI, NsiI, SbfI.


Source

aadA was isolated from a plasmid in E. coli. The original sequence was obtained from GenBank (VERSION NC_002525.1, GI:10955438).

References

GenBank.